ABI StepOnePlus Real-Time PCR

Category:
PCR Thermal Cyclers
Manufacturer:
Applied Biosystems
Listing #:
ABISTEPONEPLUSPCRMACHINE-1397
Ships Within:
3 Business Days

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The Applied Biosystems StepOnePlus is a real-time PCR system designed to meet your needs. This 96 well real-time PCR systems offer four color optical recording allowing for faster measurement.  Paired up with the software the StepOnePlus is capable of collecting melting curve data for High Resolution Melt also known as HRM.  This easy to use instrument comes with a built-in LCD touchscreen controllers allowing you to preconfigure experiments and save them in the system internal memory bank.  The StepOnePlus Real-Time PCR engineered by Lifetechnologies originally owned by Applied Biosystems (now owned by Thermo Scientific) uses a long lasting LED lighting system which has the ability to record fluorescence from SYBR Green, VIC/JOE, NED/TAMRA, FAM and works well with ROX dyes. This Real-Time PCR is extremely energy efficient and takes up minimal bench space making an ideal fit for labs who are battling for space.  


The ABI StepOne Plus Real-Time PCR comes with a built-in protocol wizard to help new users set up assays in a timely fashion and can run the full range of TaqMan assays.  This Real-Time PCR has a 99.7% accuracy with TaqMan and distinguishes populations of 5,000 and 10,000 template copies.  The StepOnePlus is a next generation Real-Time PCR built off the ever popular ABI 7500 qPCR and now the new ABI ViiA 7 technology, is a cost effective way to run a 4-color 96 well system for a fraction off the cost. Real Time PCR also called quantitative real time polymerase chain reaction (qPCR) is a technique based on PCR, which is used to amplify and at the same time quantify a targeted DNA molecule. The StepOnePlus Real-Time PCR can be used one or more specific sequences in a DNA sample, Real Time-PCR enables both detection and quantification. The quantity can be either an absolute number of copies or a relative amount when normalized to DNA input or additional normalizing genes.  This system delivers real-time PCR technology to a whole new level.  The Applied Biosystems software homepage allows you to easility navigate from one place to the next covering all phases of the Real-Time PCR process including fluorescence detection, thermal cycling and reaction set up.


The ABI StepOnePlus Real-Time PCR is a great system for scientists looking to take their genetic analysis research to the next level.  The software comes with preloaded protocols needed to run specific applications while using Applied Biosystems Reagents.  Setup takes less five minutes and you can insert your plate and go.  The Applied Biosystems Real-Time PCR software runs off of Windows XP platform and even works with Windows 7. The StepOnePlus can even assist you with selecting and ordering PCR reagents needed to run your experiment.  This Real-Time PCR has a temperature range of 4° to 100°C, accommodates 96 well plates, 0.1ml tubes and 8 tube PCR strips.  Dyes for the StepOnePlus can be purchased directly from Applied Biosystems, or Thermo Scientific and other third party vendors.  The system works well for Gene Detection and Gene Expression Profiling. Contact us today to learn more about our ABI StepOnePlus Real-Time PCR.

StepOnePlus Real-Time PCR Key Applications Include:

  • Gene Expression Profiling
  • SNP Genotyping
  • Pathogen Detection
  • Real-Time Data Tracking, Dissemination and Storage
  • MicroRNA Expression
  • Translocation Analysis
  • Viral Load Analysis
  • Gene Detection

StepOne Plus Real-Time PCR System Specifications:

Capacity

96-well plates, 0.1ml tubes

Temperature Uniformity

± 0.5c within 30 seconds of clock start

Fast Block

Yes

Excitation Source

Blue LED

Filters/Colors

4 filters factory calibrated for FAM/SYBR Green, VIC/Joe, NED/TAMRA  

Detector

Photodiodes

Demonstrated Sensitivity

10 copies

Dynamic Range

9 logs of linear dynamic range

Size (cm)

23.6 (w) x 51.2 (d) x 42.7 (h)

Multiplex Quantitation

Yes

Supported Chemistry

SYBR Green dye, TaqMan

  • Applied Biosystems StepOnePlus Real-Time PCR system
  • Windows 7 Notebook Control Workstation
  • Communication Cable
  • Power Adapter
  • Instruction Manual
  • Warranty

The StepOnePlus Real-Time PCR used for quantitative measurement was tested by our team and is performing well at factory specification.  This Real-Time PCR includes a long lasting LED optical detecion system and comes complete with a Windows 7 notebook and latests version of software already installed.  Our turnaround time from receipt of order on the StepOnePlus is three business days.  Instrument will ship out via freight will full dock-to-dock insurance.  If you would like to receive a price quote please fill out the form below and let us know if you need a lift-gate at time of delivery.  Should you have any ABI 7500 machines, ABI 7300 systems, ABI ViiA 7 instruments, or any StepOnePlus Real-Time PCR machines available for sale please let us know.  We purchased used thermal cyclers, real-time PCR, Sequencers and other genomic equipment for top dollar.  Don't let your surplus equipment go into a landfill, contact us today and we'll recycle your Real-Time PCR on to a new home.

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