ABI StepOne Plus Real Time PCR System

Description

ABI StepOne Plus Real Time PCR

The Applied Biosystems StepOne Plus is a real-time PCR system designed to meet your needs. These 96-well real-time PCR systems offer four-color optical recording allowing for faster measurement.  Paired up with the software the ABI Step One Plus is capable of collecting melting curve data for High-Resolution Melt also known as HRM.  This easy-to-use instrument comes with built-in LCD touchscreen controllers allowing you to preconfigure experiments and save them in the system’s internal memory bank.

The StepOne Plus Real-Time PCR engineered by Life Technologies, originally owned by Applied Biosystems (now owned by Thermo Scientific), uses a long-lasting LED lighting system that records fluorescence from SYBR Green, VIC/JOE, NED/TAMRA, and FAM and works well with ROX dyes. This Real-Time PCR is extremely energy efficient and takes up minimal bench space, making it an ideal fit for labs battling for space.

The StepOne Plus Real-Time PCR has a built-in protocol wizard to help new users set up assays quickly and run the full range of TaqMan assays.  This Real-Time PCR has a 99.7% accuracy with TaqMan and distinguishes populations of 5,000 and 10,000 template copies.  The StepOnePlus is a next-generation Real-Time PCR built off the ever-popular ABI 7500 qPCR and now the new ABI ViiA 7 technology, which is a cost-effective way to run a 4-color 96 well system for a fraction of the cost.

Real-time PCR also called quantitative real-time polymerase chain reaction (qPCR) is a technique based on PCR, which is used to amplify and at the same time quantify a targeted DNA molecule.  Real-time PCR can be used with one or more specific sequences in a DNA sample, and qPCR enables both detection and quantification. The quantity can be either an absolute number of copies or a relative amount when normalized to DNA input or additional normalizing genes.  This system delivers real-time PCR technology to a whole new level.  The Applied Biosystems software homepage allows you to easily navigate from one place to the next, covering all phases of the Real-Time PCR process, including fluorescence detection, thermal cycling, and reaction setup.

The ABI StepOne Plus Real-Time PCR is a great system for scientists looking to take their genetic analysis research to the next level.  The software comes with preloaded protocols for running specific applications using Applied Biosystems Reagents.  Setup takes less than five minutes; you can insert your plate and go.  The Applied Biosystems, Real-Time PCR software, runs off the Windows XP platform and even works with Windows 7.

The StepOne Plus can even assist you with selecting and ordering the PCR reagents needed to run your experiment. The system has a temperature range of 4° to 100°C and accommodates 96-well plates, 0.1ml tubes, and 8-tube PCR strips. Dyes for the StepOne Plus can be purchased directly from Applied Biosystems, Thermo Scientific, and other third-party vendors. The system works well for Gene Detection and Gene Expression Profiling. Contact us today to learn more about our ABI StepOne Plus Real-Time PCR.